Glycoforms In Health & Disease - The Science
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The glycans on glycoproteins contribute to protein function through properties such as stability and interactions.
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All current tumor markers (e.g. CA125, PSA) are glycoproteins but current assays do not measure the glycans on the proteins. Studies show improved accuracy when glycoforms are measured.
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Altered glycans and glycoforms are observed during the development of diseases such cancers, inflammatory and autoimmune conditions.
Illustration of two glycoforms of one glycoprotein
Glycoprotein = protein with sugar(s) attached.
Glycoform = a variant of glycoprotein defined by the particular sugars.
Glycan = scientific term for sugar.
Lectin Magnetic Bead Array-mass Spectrometry (LeMBA-MS) Platform For Blood Serum Glycoform Biomarker panel discovery
Design Features
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Streamlined glycoform enrichment with in-built quality control
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Spiked internal control protein added to each serum sample for quality assurance during LeMBA.
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Liquid handler pulldown and on-bead tryptic digest for throughput and consistency.
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Single platform from research to clinical
LeMBA is coupled to appropriate MS techniques: data-dependent acquisition (DDA) or data-independent acquisition (DIA) for discovery, multiple reaction monitoring (MRM) for validation and clinical assay.
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Rapid deployment to clinical MS assay
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No new reagent to be developed.
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Reduces the time, cost and risk of product development.
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Diagnostic panel with algorithm increases accuracy
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Algorithm will be developed to interpret probability of ovarian cancer.
Proof of concept completed: esophageal adenocarcinoma biomarkers licensed to Proteomics International in June 2022.
